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URL: http://www.addgene.org/35538
Proper Citation: RRID:Addgene_35538
Insert Name: 3'UTR of ZEB2 gene
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:18376396
Vector Backbone Description: Backbone Marker:Witold Filipowicz; Backbone Size:6380; Vector Backbone:pCI-neo-RL; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Comments: Mutations in the miR-200b seed regions of the ZEB2 3'UTR were generated using the QuikChange Multi site-directed mutagenesis kit. The ZEB2 3'UTR was cloned into the plasmid pCIneo-RL which was obtained from Witold Filiowicz. Pillai R, Bhattacharyya S, Artus C, Zoller T, Cougot N, Basyuk E, Bertrand E, Filipowicz W (2005) Inhibition of translational initiation by let-7 microRNA in human cells. Science 309:1573-1576 Please note that Addgene's sequencing results identified a single nucleotide deletion at bp# 2440 when compared to the full plasmid sequence provided by the depositing laboratory. Addgene's sequencing results are correct as the extra base in the sequence from the depositing laboratory was an error due to a sequencing artifact.
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Source: Addgene
