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URL: http://www.addgene.org/35688
Proper Citation: RRID:Addgene_35688
Insert Name: loxP-STOP-loxP
Organism: SV40
Bacterial Resistance: Kanamycin
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3500; Vector Backbone:pCR-Blunt II-TOPO; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Kanamycin
Comments: If you are using the cassette for gene targeting, you should check the number of SV40 repeats in the targeted locus as the lab has noticed that in some ES clones copies of the repeats were missing. Please also see Lox-Stop-Lox TOPO (http://www.addgene.org/11584/) for a puromycin version of this plasmid and to download a document about using the Lox-Stop-Lox system.
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Source: Addgene
