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URL: http://www.addgene.org/36256
Proper Citation: RRID:Addgene_36256
Insert Name: lacZ
Organism: E. coli
Bacterial Resistance: Kanamycin
Defining Citation: PMID:22279533
Vector Backbone Description: Backbone Marker:Stover et al., 1991 Nature 351:456; Vector Backbone:pMV261; Vector Types:Bacterial Expression, Synthetic Biology, Mycobacteria replicating vector; Bacterial Resistance:Kanamycin
Comments: The plasmid is high copy in E. coli, but low copy in mycobacteria. Cloning was done via assembly PCR, sot he XbaI-HindIII fragment comprises the promoter-riboswitch. The insert is 3361bp including the promoter. There are mismatches between Addgene's quality control sequence and the depositor sequence, but they are not known to affect plasmid function.
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Source: Addgene
