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Plasmid Name
pcDNA-ER-ZapCY1
RRID:Addgene_36321 RRID Copied  
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RRID:Addgene_36321
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Plasmid Information

URL: http://www.addgene.org/36321

Proper Citation: RRID:Addgene_36321

Insert Name: ER-ZapCY1 high affinity Zn(II) sensor

Organism: Synthetic

Bacterial Resistance: Ampicillin

Defining Citation: PMID:21502528

Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin

Comments: Second generation genetically encoded, ratiometric, fluorescent biosensor. Zn(II)-binding domain derived from the first 2 zinc fingers of Zap 1 from Saccharomyces cerevisiae. Contains truncated CFP and Citrine fluorescent proteins. Targeted to ER: contains C-terminal KDEL

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Data and Source Information

Source: Addgene