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URL: http://www.addgene.org/36422
Proper Citation: RRID:Addgene_36422
Insert Name: BCR
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:19840940
Vector Backbone Description: Backbone Marker:p CD from Paul Berg; Backbone Size:3000; Vector Backbone:pCDE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Construct includes 5' untranslated sequences, and the first 39 aminoacids including a small part of the N-terminal oligomerization domain. The deletion was generated by ligation of a Stu I site in BCR exon 1 to a downstream Nae I site. The construct lacks the ST kinase domain. Vector is a derivative of pCD with a multiple cloning site included. Has SV-40 origin of replication for high yield expression in COS-1 cells. Also see Yi et al 2009. Constructed by Vesa Kaartinen.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
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