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URL: http://www.addgene.org/36449
Proper Citation: RRID:Addgene_36449
Insert Name: human SP-C promoter
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:22482505
Vector Backbone Description: Backbone Marker:Richard Mulligan; Backbone Size:4874; Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: Cloned h3.7kb SP-C promoter (from Whitsett 3.7SPC/SV40 plasmid) into pHAGE-CMV-GFP-W as follows: SV40t removed from SPC plasmid by BamH1-BamH1 cutting, blunting and self-ligtion of blunt ends. Entire hSPC promoter lifted out by cutting Nde1-Not1 and ligated into pHAGE CMV-GFP-W backbone pre-cut Nde1-Not1 to remove most of CMV promoter. Cut this backbone Spe1-Nde1 to remove residual CMV promoter fragment, blunted and ligated NB: final vector still has Spe1 site at start of promoter.
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Source: Addgene
