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URL: http://www.addgene.org/37164
Proper Citation: RRID:Addgene_37164
Insert Name: ~1200-bp fragment from Burkholderia xenovorans LB400 cloned into pEXT20
Organism: Burkholderia xenovorans
Bacterial Resistance: Ampicillin and Kanamycin
Defining Citation: PMID:18326575
Vector Backbone Description: Vector Backbone:pUX2442; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This strain contains Roberts lab plasmid pUX2442 which contains ~1200bp fragment from Burkholderia xenovorans LB400 with introduced terminal HindIII and SalI sites [such that HindIII – (<-rcoM1 – intergenic region – coxM1′->) – SalI] cloned into pEXT20 with the lacZ + KanR cassette from pKOK6 cloned at the SalI site such that coxM1′::lacZ->tfd<-KanR. Suitable template for amplification of the Burkholderia xenovorans RcoM1 binding region + reporter cassette. For strain usage see attached pdf.
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Source: Addgene
