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URL: http://www.addgene.org/37167
Proper Citation: RRID:Addgene_37167
Insert Name: coxM1′::lacZ reporter system AND Burkholderia xenovorans RcoM1 with C-term 6xHIS tag
Bacterial Resistance: Ampicillin and Kanamycin
Vector Backbone Description: Vector Backbone:pUX2996 and pUX2410; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX2996 (pUX2970 with a coxM1′::lacZ reporter system such that ...vector DNA – BamHI (unique site) – “e + f” binding region – coxM1′(with unique SalI site)::lacZ-> tfd <-KanR (with 5′-end unique XhoI site) – vector DNA <-SpR...) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag). Strain is CmR, plasmid pUX2996 is KanR and SpecR and plasmid is pUX2410 is AmpR. Can be grown in media containing Amp + Kan. For strain usage see attached pdf. Please note that this strain has not been tested functionally by Addgene.
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Source: Addgene
