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URL: http://www.addgene.org/37178
Proper Citation: RRID:Addgene_37178
Insert Name: coxM1′::lacZ reporter system with RcoM binding sites "a-f" spaced at 21bp AND RcoM1 with C-term 6xHIS tag
Bacterial Resistance: Ampicillin and Kanamycin
Vector Backbone Description: Vector Backbone:pUX3254 AND pUX2410; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX3254 (coxM1′::lacZ reporter system with binding sites “a-f” with a deletion between the “c” and “d” binding motifs such that the individual binding motifs “a,” “b,” “c,” “d,” “e,” and “f” are spaced at 21-bp intervals) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag). Strain is CmR, pUX3254 is KanR and SpecR, pUX2410 is AmpR. Can be grown in Amp + Kan. For strain usage see attached pdf.
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Source: Addgene
