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Plasmid Name
pDonor MCS Rosa26
RRID:Addgene_37200 RRID Copied  
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RRID:Addgene_37200
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Plasmid Information

URL: http://www.addgene.org/37200

Proper Citation: RRID:Addgene_37200

Insert Name: Rosa26 left targeting arm

Organism: Mus musculus

Bacterial Resistance: Ampicillin

Defining Citation: PMID:22169954

Vector Backbone Description: Backbone Marker:Sigma; Vector Backbone:pZDonor AAVS1; Vector Types:Genomic targeting - zinc finger; Bacterial Resistance:Ampicillin

Comments: The homology arms in the ROSA26 donor vector were generated by PCR of mouse genomic DNA and insertion of the PCR-amplified right homology arm (left primer: 5-TTATTGCGGCCGCAG ATGGGCGGGAGTCTTCT-3, right primer: 5-AACA CCGCGGCAGTTTATAAATGGAGAAAAAGGAGA -3) between the NotI and SacII sites and the left homology arm (left primer: 5-TCTATGGTACCAGTTAACGGCA GCCGGAGT-3 , right primer: 5 -CGGACGAATTCTCT AGAAAGACTGGAGTTGCAGA-3) between the KpnI and EcoRI sites in the pZDonor AAVS1 vector obtained from Sigma–Aldrich.

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Data and Source Information

Source: Addgene