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URL: http://www.addgene.org/37535
Proper Citation: RRID:Addgene_37535
Insert Name: ss-cfSGFP2 (cysteine-free SGFP2)
Organism: Homo sapiens
Bacterial Resistance: Kanamycin
Defining Citation: PMID:22649538
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4105; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: This plasmid is used for expression of cfSGFP2 in the ER. The cleaved signal sequence of α1-antitrypsin (M1-K34) was fused to the N-terminus of SGFP2 using BglII and EcoRI sites. The fragment M1-A24 should be removed cotranslationally after translocation. The long flanking sequence E25-K34 of α1-antitrypsin was included to ensure access to signal peptidase
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Source: Addgene
