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URL: http://www.addgene.org/38207
Proper Citation: RRID:Addgene_38207
Insert Name: Bxb1-Excisionase
Bacterial Resistance: Chloramphenicol
Defining Citation: PMID:22615351
Vector Backbone Description: Backbone Size:2800; Vector Backbone:J64100; Vector Types:Synthetic Biology, BioBrick cloning vector; Bacterial Resistance:Chloramphenicol
Comments: In the reset circuit, PBAD controls expression of a polycistron encoding excisionase with a strong RBS followed by Bxb1 integrase with a GTG start codon to decrease its translation rate and increase the excisionase to integrase ratio. This construct was cloned in a plasmid with a regulated ColE1 origin of replication (J64100, 50-70 copies, chloramphenicol resistance).
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Source: Addgene
