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URL: http://www.addgene.org/39209
Proper Citation: RRID:Addgene_39209
Insert Name: KOG1
Organism: Saccharomyces cerevisiae
Bacterial Resistance: Ampicillin
Defining Citation: PMID:12408816
Vector Backbone Description: Backbone Size:6100; Vector Backbone:YCplac111; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Expresses 2x-myc-tagged KOG1 under control of KOG1 promoter. The two myc-tags are integrated between codons 1 and 2 of KOG1. A 6.0 kb PCR fragment containing the KOG1 locus (−972 to +304 with respect to the ORF) was cloned into the XbaI and Asp718I sites of YCplac111; the sequence ATG ATC CCC GGG TTA ATT AAC GGT GAA CAA AAG CTA ATC TCC GAG GAA GAC TTG AAC GGT GAA CAA AAA TTA ATC TCA GAA GAA GAC TTG AAC GGA CTC GAC GGT GAA CAA encoding two myc tags was integrated between codons 1 and 2 of KOG1 Addgene's sequencing result identified a L1512P mutation in the KOG1 sequence when compared to GenBank reference sequence NP_012056.1. The depositing laboratory confirmed the plasmid is functional as described in the associated publication.
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No rating or validation information has been found for YCplac111-KOG1 (pAN58).
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Source: Addgene
