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URL: http://www.addgene.org/40122
Proper Citation: RRID:Addgene_40122
Insert Name: Mex-5 (ATG through 648 bp of 3'UTR) (RNAi resistant exon 2)
Organism: Caenorhabditis elegans
Bacterial Resistance: Kanamycin
Defining Citation: PMID:21925318
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4773; Vector Backbone:pDONR P2RP3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Comments: A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene. Alternate plasmid name: pDONR P2RP3+MEX-5 genomic(RNAI res exon 2) + 3’UTR
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Source: Addgene