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URL: http://www.addgene.org/40281
Proper Citation: RRID:Addgene_40281
Insert Name: 2C Promoter (2-cell stage promoter)
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:22722858
Vector Backbone Description: Backbone Marker:Invitrogen/Life Tech; Backbone Size:6400; Vector Backbone:pcDNA Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The CMV promoter from the parent pcDNA plasmid was removed with MluI and HindIII, leaving approx. 6400kb backbone. The 2C promoter was put in its place (730bp) using the same sites. Hence, the full plasmid is 7129bp. The promoter is active shortly after fertilization of mouse embryos to about the 8-cell stage. It is also on in a subset of MESCs & miPSCs.
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Source: Addgene
