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URL: http://www.addgene.org/40832
Proper Citation: RRID:Addgene_40832
Insert Name: Two target sites perfectly complementary to miR-1
Organism: Drosophila melanogaster
Bacterial Resistance: Ampicillin
Defining Citation: PMID:20558712
Vector Backbone Description: Backbone Marker:Murhpy Lab, Carnegie Institution for Science; Backbone Size:7233; Vector Backbone:pAW; Vector Types:Insect Expression, miRNA reporter; Bacterial Resistance:Ampicillin
Comments: The miR-1 complimentary sequence was inserted in the XbaI site in the 3'UTR of EGFP using the following oligos: s: CTA GAC TCC ATA CTT CTT TAC ATT CCA TCT AGA CTC CAT ACT TCT TTA CAT TCC AT as: CTA GAT GGA ATG TAA AGA AGT ATG GAG TCT AGA TGG AAT GTA AAG AAG TAT GGA GT This expression cassette was then sub-cloned into the Gateway vector pAW using GATEWAY cloning.
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No rating or validation information has been found for pAW-EGFP-2xmiR-1 (pAW-AS-21).
No alerts have been found for pAW-EGFP-2xmiR-1 (pAW-AS-21).
Source: Addgene
