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URL: http://www.addgene.org/41161
Proper Citation: RRID:Addgene_41161
Insert Name: PBD HK-AA
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:16267267
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1/3x myc-C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Addgene plasmid #41162: Plk1-PBDWT (aa 326–603) was cloned in-frame by PCR into a pcDNA3.1 vector encoding an amino-terminal 3xmyc-tag (Invitrogen, Carlsbad, CA) using primers 5'-CCG GAA TTC CAG ATC TTC GAT TGC TCC CAG CAG CCT GG-3' and 5'-CCG CTC GAG TTA GGA GGC CTT GAG ACG GTT GC-3'. The Plk1-PBDAA mutant (H538A, K540A) was made by site-directed mutagenesis using the following primers: 5'-CAA TTC TCC GGA GCC CCG CCT ATC TGT CCC-3' and 5'-GGG ACA GAT AGG CGG GGC TCC GGA GAA TTG-3'.
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No rating or validation information has been found for pcDNA3 PBD HK-AA (Nigg AH13).
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Source: Addgene
