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URL: http://www.addgene.org/43917
Proper Citation: RRID:Addgene_43917
Insert Name: GCaMP3
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:22174877
Vector Backbone Description: Backbone Marker:Addgene plasmid 43915; Backbone Size:9600; Vector Backbone:FU-MAP2-Gateway; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: The MAP2-GFP lentiviral reporter plasmid pGZ-hMAP2 was purchased from System Biosciences (SR10047PA-1). The MAP2 promoter sequence from this plasmid was then cloned along with the Gateway cassette of pEF-DEST51 into the backbone of FUdeltaGW-rtTA (Addgene plasmid 19780) following removal of the ubiquitin promoter-rtTA sequence to produce FU-MAP2-Gateway (Addgene plasmid #43915). This destination vector was subsequently used to generate MAP2-GCaMP3 (GCaMP3 cloned from G-CaMP3, Addgene plasmid 22692). The V5 and His tags present in the vector backbone are not expressed with the GCaMP3 insert.
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Source: Addgene
