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URL: http://www.addgene.org/45287
Proper Citation: RRID:Addgene_45287
Insert Name: TLOC1
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:23764425
Vector Backbone Description: Vector Backbone:pLEX_970; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: The initial pDONR223 clone was obtained from the CCSB Human Orfeome Collection. Upon sequencing it was found that the construct contained a 660 bp long variant as compared to the expected 1200 bp long version. To determine whether this shorter version is expressed in cancer cells, and to exclude the possibility of this being a cloning artifact, we cloned TLOC1 from T47D cells and found both forms to be expressed. We re-cloned both the full length and shorter splice variants by ligation of BP flanked PCR products into pDONR223 by a Gateway BP reaction. TLOC1 was transferred into DEST vectors from TLOC1 containing pDONR223 vectors through a Gateway LR reaction.
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No rating or validation information has been found for pLEX_970_puro_DEST_V5_TLOC1 (220aa).
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Source: Addgene
