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URL: http://www.addgene.org/45443
Proper Citation: RRID:Addgene_45443
Bacterial Resistance: Chloramphenicol and Ampicillin
Defining Citation: PMID:18754012
Vector Backbone Description: Backbone Marker:Connie Cepko lab (Harvard Medical School, Boston, MA); Backbone Size:7349; Vector Backbone:pEF-GFP (Addgene Plasmid #11154); Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Alternate plasmid name: pEF1α-RfC.1-IRES-GFP To construct this vector, the depositing lab used EcoRI and BsrGI to replace the EGFP in pEF–GFP (Addgene plasmid 11154) with the IRES-GFP fragment from plasmid pBMN-I-GFP (Addgene plasmid 1736), creating intermediate vector pEF1α-IRES-GFP. Plasmid pEF1α-IRES-GFP was then converted to a Gateway-enabled destination vector with the Gateway Vector Conversion System (Invitrogen) by digesting with EcoRI, blunting the ends, and inserting Reading Frame Cassette C (RfC.1) gateway cassette.
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Source: Addgene
