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URL: http://www.addgene.org/45634
Proper Citation: RRID:Addgene_45634
Insert Name: AT1AR-TSTS/A
Organism: Rattus norvegicus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:15355986
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA3.1/Zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Mutant AT1A receptors were generated by mutagenesis PCR using pcDNA3.1-HA-AT1A receptor (provided by M. G. Caron, Duke University) as a template and a QuikChange multisite-directed mutagenesis kit (Stratagene) according to the manufacturer’s instructions. The primer for the TSTS/A receptor was: 5' p-CTCAAGCCTGTCTGCGAAAATGGCCGCTTGCTTACCGGCCTTC 3'. N4D mutation introduced to prevent glycosylation at this consensus site and steric hindrance of antibody binding.
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Source: Addgene
