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Plasmid Name
pcDNA3 HA eIF4GI (1-1599)
RRID:Addgene_45640 RRID Copied  
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RRID:Addgene_45640
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Plasmid Information

URL: http://www.addgene.org/45640

Proper Citation: RRID:Addgene_45640

Insert Name: eIF4GI transcript variant 2

Organism: Homo sapiens

Bacterial Resistance: Ampicillin

Defining Citation: PMID:19114555

Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin

Comments: For diagnostic digest, an additional PvuI digestion is necessary to distinguish the insert from the vector because of their similar sizes. The EIF4G1 translation contains M432V compared to NP_937884 (NM_198241.2); however the nucleotide change resulting in the amino acid change is more conserved between species, and it is located between functional domains of eIF4G1 (PABP-binding site and eIF4E-binding site), and does not affect its function as a translational factor

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Data and Source Information

Source: Addgene