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URL: http://www.addgene.org/45893
Proper Citation: RRID:Addgene_45893
Insert Name: Nck-1 SH2 mutant
Organism: Homo sapiens
Bacterial Resistance: Kanamycin
Defining Citation: PMID:11959995
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Wild type pEGFP-Nck1 (Addgene plasmid 45903) is described in JBC 26:26633-44 2006 (PMID 16835242). Mutations are described in the article linked to this plasmid (PMID 11959995) and then subcloned into the pEGFP-C1 vector using PCR amplification with primers containing XhoI and EcoRI sites.
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Source: Addgene
