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URL: http://www.addgene.org/46276
Proper Citation: RRID:Addgene_46276
Insert Name: Vcl 1-1066 + EYFP
Organism: Gallus gallus
Bacterial Resistance: Kanamycin
Defining Citation: PMID:15883197
Vector Backbone Description: Backbone Marker:Craig lab; Vector Backbone:pECFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: A 9-bp fragment encoding a NotI site was introduced by mutagenesis into pEGFP-C1/vinculin cDNA (chicken) immediately after the codon for aa 883 (Coutu and Craig, 1988) using the Quick Change kit (Stratagene). The cDNA of EYFP (CLONTECH Laboratories, Inc.) minus the stop codon and flanked by NotI was inserted into NotI-digested vinculin.
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Source: Addgene