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URL: http://www.addgene.org/47032
Proper Citation: RRID:Addgene_47032
Bacterial Resistance: Chloramphenicol and Ampicillin
Defining Citation: PMID:23827685
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4349; Vector Backbone:pMT/BiP/V5/His A; Vector Types:Insect Expression, Secreted expression in Drosophila Culture; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Inducible with 0.8 uM Copper Sulfate. Targets proteins to the extracellular millieu using the Drosophila melanogaster BiP signal peptide. Expresses in Drosophila melanogaster cell lines, such as Schneider 2 (S2) cells. Published in Ozkan et al, Cell 154(1): 228 (2013); PMID: 23827685; doi: 10.1016/j.cell.2013.06.006 After recombination, transform and grow in DH5alpha. The plasmid is no longer chloramphenicol resistant after recombination.
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Source: Addgene
