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URL: http://www.addgene.org/47683
Proper Citation: RRID:Addgene_47683
Insert Name: TP-1 promoter
Bacterial Resistance: Ampicillin
Defining Citation: PMID:16508309
Vector Backbone Description: Backbone Marker:described in Kato, et al. 1997 PMID 9374409 ; Vector Backbone:pGA981-6; Vector Types:Notch reporter construct; Bacterial Resistance:Ampicillin
Comments: This plasmid was constructed from the pGa981-6 plasmid by replacing the luciferase gene in the original pGA981-6 plasmid with dsRedExpress (Clontech) using XhoI/SmaI sites. The construction of pGA981-6 was described as: "Six copies of this oligonucleotide were cloned in the BamHI site: 5' strand GATCCCGACT CGTGGGAAAA TGGGCGGAAG GGCACCGTGG GAAAATAGTA............ 3' strand ............GGCTGA GCACCCTTTT ACCCGCCTTC CCGTGGCACC CTTTTATCAT CTAG" Please note that Addgene's sequencing results indicate that the first and fourth ERE-TP1 repeats differ from the above oligo sequence at the twelfth nucleotide (G->A). These differences may have occurred during construction of the original pGA981-6 plasmid.
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Source: Addgene
