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URL: http://www.addgene.org/47802
Proper Citation: RRID:Addgene_47802
Insert Name: PesaR-luxCDABE-term
Bacterial Resistance: Ampicillin
Defining Citation: PMID:23879176
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:2934; Vector Backbone:pET-17b; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: PesaR-luxCDABE-transcriptional terminator fragment from pCS-PesaRlux is between XhoI and BglII sites in pET17b. PesaR (274bp, indicated as lowercase letters in the 2nd partial sequence for PesaR between XhoI and BamHI) is located between XhoI and BamHI sites. luxCDABE is located between two NotI sites. The transcriptional terminator is located downstream of luxCDABE between the second NotI and BglII. Sequencing primers provided were used to sequence PesaR.
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Source: Addgene