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URL: http://www.addgene.org/47946
Proper Citation: RRID:Addgene_47946
Insert Name: Cas9
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:23979586
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2958; Vector Backbone:pBluescript SK+; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: To generate the unique SpeI site, a short, 37 bp, linker was inserted into an existing NdeI site. This linker seems to have been inserted 2 or more times, creating a short repeat region that we have been unable to sequence across. Sequencing with the M13 reverse primer will fail.
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Source: Addgene
