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URL: http://www.addgene.org/48999
Proper Citation: RRID:Addgene_48999
Insert Name: Recoded E. coli genome with all instances of the UAG codon removed, but wild type UAG termination function is not changed
Organism: E. coli
Bacterial Resistance: Ampicillin
Defining Citation: PMID:24136966
Vector Backbone Description: Vector Backbone:E. coli genome; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: E. coli MG1655 delta(ybhB-bioAB)::[lcI857 N(cro-ea59)::tetR-bla] Delta mutS::zeoR; all 321 UAG codons changed to UAA This strain is mutS-, which causes a spontaneous mutation frequency of ~2E-8. This strain is auxotrophic for d-biotin. Sequence is similar to C321.DA (NCBI accession # NCBI CP006698), except that RF1 is not deleted.
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Source: Addgene
