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URL: http://www.addgene.org/49184
Proper Citation: RRID:Addgene_49184
Bacterial Resistance: Ampicillin
Defining Citation: PMID:24215641
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Note that there are two adenines in Addgene's T7 quality control sequence that introduce a HindIII site and an AflII site into the plasmid. Note that these discrepancies with the depositor sequence do NOT affect the function of the plasmid. The vector is used as a template for PCR to amplify DNA cassettes to modify chromosomal gene loci. The additional adenines are not within the primer sequences to amplify DNA cassettes for gene manipulation.
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Source: Addgene
