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URL: http://www.addgene.org/49390
Proper Citation: RRID:Addgene_49390
Insert Name: Sox2
Organism: Homo sapiens
Bacterial Resistance: Chloramphenicol and Kanamycin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4900; Vector Backbone:pLPS-3'EGFP; Vector Types:Mammalian Expression, Cre/Lox, acceptor vector; Bacterial Resistance:Chloramphenicol and Kanamycin
Comments: hSox2 was transferred from the donor vector (pDNR-hSox2, Addgene plasmid #49389) to the acceptor vector using Cre-mediated recombination at the LoxP sites. When the recombinant construct is expressed in mammalian cells, EGFP is expressed from the immediate early promoter of cytomegalovirus (pCMV IE) as a 3' fusion to Sox2, through intron splicing.
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Source: Addgene
