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URL: http://www.addgene.org/51675
Proper Citation: RRID:Addgene_51675
Insert Name: HIS3
Organism: Saccharomyces cerevisiae
Bacterial Resistance: Ampicillin
Defining Citation: PMID:12898713
Vector Backbone Description: Vector Backbone:YDp-K; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin
Comments: Plasmid D588 (lys2::HIS3 ) was constructed in two steps. First, plasmid YDp-K was cut with BglII and XhoI and ligated, deleting a 2477 bp fragment inside the LYS2 gene. This plasmid was then cleaved with BglII and a 1.2 kb BamHI fragment from YDp-H containing HIS3 was inserted, creating D558.
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Source: Addgene
