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URL: http://www.addgene.org/51677
Proper Citation: RRID:Addgene_51677
Insert Name: LYS2
Organism: Saccharomyces cerevisiae
Bacterial Resistance: Ampicillin
Defining Citation: PMID:12898713
Vector Backbone Description: Vector Backbone:pIC19H–SXL2; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin
Comments: Plasmid D771 (leu2::LYS2) was constructed in two steps. First, plasmid YDp-K was cut with SalI and an EcoRI linker was inserted, constructing plasmid D764. Next, plasmid pIC19H–SXL2 was cut with EcoRI and a 5 kb EcoRI fragment containing LYS2 from plasmid D764 was inserted, creating D771.
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Source: Addgene