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Plasmid Name
D771 leu2::LYS2 Disruptor Converter
RRID:Addgene_51677 RRID Copied  
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RRID:Addgene_51677
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Plasmid Information

URL: http://www.addgene.org/51677

Proper Citation: RRID:Addgene_51677

Insert Name: LYS2

Organism: Saccharomyces cerevisiae

Bacterial Resistance: Ampicillin

Defining Citation: PMID:12898713

Vector Backbone Description: Vector Backbone:pIC19H–SXL2; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin

Comments: Plasmid D771 (leu2::LYS2) was constructed in two steps. First, plasmid YDp-K was cut with SalI and an EcoRI linker was inserted, constructing plasmid D764. Next, plasmid pIC19H–SXL2 was cut with EcoRI and a 5 kb EcoRI fragment containing LYS2 from plasmid D764 was inserted, creating D771.

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Data and Source Information

Source: Addgene