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Plasmid Name
M4786 KanMX::hisG-URA3-HisG Disruptor Converter
RRID:Addgene_51690 RRID Copied  
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RRID:Addgene_51690
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Plasmid Information

URL: http://www.addgene.org/51690

Proper Citation: RRID:Addgene_51690

Insert Name: hisG-URA3-hisG

Organism: Saccharomyces cerevisiae

Bacterial Resistance: Ampicillin

Defining Citation: PMID:12898713

Vector Backbone Description: Vector Backbone:M4757 (kanMX::TRP1), Addgene plasmid #51687; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin

Comments: M4786 (kanMX::hisG-URA3-hisG) was made by digesting M4757 with BamHI and SalI and inserting a 3.9 kb BamHI–SalI fragment with hisG–URA3–hisG from plasmid M2524. The kanMX::hisG–URA3–hisG converter is recyclable, so that any MX-based disruption can be reverted to an unmarked disruption, allowing reuse of markers.

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Data and Source Information

Source: Addgene