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URL: http://www.addgene.org/51710
Proper Citation: RRID:Addgene_51710
Bacterial Resistance: Ampicillin
Defining Citation: PMID:16278461
Vector Backbone Description: Vector Backbone:pBluescript KS+; Vector Types:T. brucei genome integration vectors; Bacterial Resistance:Ampicillin
Comments: The PTP tag is a derivative of the original TAP tag and has CBP replaced by ProtC. Gene flanks providing RNA processing signals for PTP fusion and resistance marker are the 3′ flank of TbRPA1 (RPA1-3′), HSP70 genes 2 and 3 intergenic region (H23), the β-α tubulin intergenic region (T), and the 5′ flank of TbRPA2 (RPA2-5′). pC-PTP-NEO contains a target sequence (U1-70K) which can be replaced by the gene of interest.
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Source: Addgene
