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Plasmid Name
rEc 27.H.Sc
RRID:Addgene_51948 RRID Copied  
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RRID:Addgene_51948
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Plasmid Information

URL: http://www.addgene.org/51948

Proper Citation: RRID:Addgene_51948

Bacterial Resistance: Spectinomycin

Defining Citation: PMID:25188631

Vector Backbone Description: Vector Backbone:NA; Vector Types:; Bacterial Resistance:Spectinomycin

Comments: LB Lennox, at 32°C or below. Strain has a biotin auxotrophy, but this should not affect growth in rich media. It has 13 "essential" genes that end in TAG recoded to TAA, and its prfA gene has been deleted. Though viable, strain growth is slow. Genotype: ΔmutS:cat.Δ(ybhB-bioAB):[λcI857.Δ(cro-ea59):tetR-bla], ΔprfA, spec+

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Data and Source Information

Source: Addgene