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URL: http://www.addgene.org/52989
Proper Citation: RRID:Addgene_52989
Insert Name: OxyS sRNA
Organism: E.coli
Bacterial Resistance: Ampicillin
Defining Citation: PMID:21189298
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: The transcription of each sRNA was controlled by the pLlacO-1 promoter, which was induced by adding isopropyl-β-d-thiogalactopyranoside (IPTG) to the media. The part of the target mRNA that is necessary for sRNA regulation was fused to gfp and constitutively transcribed from the pLtetO-1 promoter without TetR in the system. GFP fluorescence provides a quantitative measure of target gene expression and sRNA activity.
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Source: Addgene
