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URL: http://www.addgene.org/53635
Proper Citation: RRID:Addgene_53635
Insert Name: SP100A
Organism: Other
Bacterial Resistance: Kanamycin
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pSUMO-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-SUMO tags, SUMO protease, LIC cloning using BsaI digest/T4 polymerase but non-standard LIC sites, SacB stuffer fragment. Optional non-cleavable biotinylation and His6 tags if reverse primer does not encode stop codon. NB. SUMO recognition sequence is a dummy entry. http://www.thesgc.org/structures/4PTB/ Use BL21(DE3)-R3-pRARE2 strain for expression.
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Source: Addgene
