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URL: http://www.addgene.org/59766
Proper Citation: RRID:Addgene_59766
Insert Name: CAS9
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:23997119
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The eGFP ORF was inserted into C-terminal of CAS9 ORF of pCAG-T3-hCAS-pA (Addgene Plasmid #48625). For the in vitro synthesis of CAS9 mRNAs, the vector was linearized by SphI and in-vitro transcribed using T3-RNA-polymerase (Promega) in the presence of m7G(5′)ppp(5′)G to synthesize capped RNA. The Tbpl1 3′UTR 95 nucleotide polyA tail at the 3' end of the insert allows in vitro transcribed mRNA from this plasmid to be used directly for microinjection into animal embryos or oocytes without additional polyadenylation treatment. The CAG promoter in this plasmid also allows it to be used as a CAS9eGFP-expression vector.
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Source: Addgene
