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URL: http://www.addgene.org/60410
Proper Citation: RRID:Addgene_60410
Insert Name: SspB micro
Organism: E. Coli
Bacterial Resistance: Ampicillin
Defining Citation: PMID:25535392
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:5979; Vector Backbone:pQE-80L; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The SspB used in this construct contains two amino acid mutations that disrupt the natural dimerization of SspB. These mutations are Y11K and A15E (residue numbering from pdb code 1ou9). Here is the sequence around the mutated residues with the sites of mutation in parentheses: SSPKRP(K)LLR(E)YYDWLVDNS
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Source: Addgene
