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URL: http://www.addgene.org/61765
Proper Citation: RRID:Addgene_61765
Insert Name: Hygromycin
Organism: Synthetic
Bacterial Resistance: Kanamycin
Defining Citation: PMID:26092796
Vector Backbone Description: Backbone Marker:CAMBIA; Backbone Size:6812; Vector Backbone:pCAMBIA-0380; Vector Types:Ternary vector for Agrobacterium mediated transformation of fungi; Bacterial Resistance:Kanamycin
Comments: Jason Rudd (Rothamsted research UK) provided Agrobacterium tumfaciens vector pCHYG. For more information, see: Motteram et al, 2009. Molecular Plant-Microbe Interactions, 22, 790-799. https://www.ncbi.nlm.nih.gov/pubmed/19522561. Restriction sites EcoRI and HindIII can be used to linearise the vector inside left (LB) and right (RB) borders of the TDNA. Note that the trpC promoter and hygromycin sequences are present within the EcoRI and HindII cloning sites. See Supplementary Fig. S1. of associated publication (Sidhu et al., 2015) for schematic of single step assembly of gene deletion ternary vectors using yeast recombinational cloning.
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Source: Addgene
