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URL: http://www.addgene.org/62232
Proper Citation: RRID:Addgene_62232
Insert Name: hamster BiP-mGFP-KDEL
Organism: Synthetic
Bacterial Resistance: Kanamycin
Defining Citation: PMID:20410136
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4000; Vector Backbone:clontech N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Avoid overexpression. Low expressing cells are best for imaging. High expression leads to protein aggregation in the nuclear envelope. See supplementary data for original paper. This mutant can not bind client proteins. Do NOT use this as an ER marker. BiP is a functional protein and significantly impacts the ER environment increasing stress resistance. Use ER-GFP if you simply want a marker for the ER when imaging cells.
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Source: Addgene
