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URL: http://www.addgene.org/63089
Proper Citation: RRID:Addgene_63089
Insert Name: expanded CGG repeats (99 repeats) within the 5'UTR of FMR1
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Should be amplified in STBL3 bacteria and grown at Room temperature to keep the repeats stable. This construct allows translation of CGG repeats into 99 glycine due to a near cognate ATG codon upstream of the repeat in the 5'UTR of FMR1 (an ACG codon that initiates weakly translation). This plasmid should be verified via restriction analysis. Multiple colonies may need to be screened to isolate the correct construct.
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Source: Addgene
