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URL: http://www.addgene.org/63575
Proper Citation: RRID:Addgene_63575
Insert Name: Col1A-frt-hygro-pA
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:26492136
Vector Backbone Description: Backbone Size:2888; Vector Backbone:pBluescriptII SK; Vector Types:Mammalian Expression, Bacterial Expression, Mouse Targeting, FRT/Flpe; Bacterial Resistance:Ampicillin
Comments: The cassette in between the homologous recombination arms is the same as in AddGene Plasmid #20730. The homologous recombination arms are derived from mouse strain FVB. The Hygromycin gene in this construct does not have an ATG start codon nor is it expressed. Hygromycin can not be used for selection when this construct is used in a targeting experiment in mouse strain FVB derived ES cells. The construct can be linearized with AscI, FseI or XhoI
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Source: Addgene
