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URL: http://www.addgene.org/64049
Proper Citation: RRID:Addgene_64049
Insert Name: GLUT4
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:25575350
Vector Backbone Description: Backbone Size:9124; Vector Backbone:pLenti-hiko; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: The c-Myc sequence was inserted into the region encoding the 1st exofacial loop of mouse GLUT4 in the background of pmCherry-N1. The fragment of Myc-GLUT4-mCherry was then sub-cloned into pLenti-hiko vector via NheI + HpaI digestion. Discrepancies between the Addgene QC sequence and the full sequence are not of functional concern. Please cite the following article: Lim, C.-Y. et al. Tropomodulin3 is a novel Akt2 effector regulating insulin-stimulated GLUT4 exocytosis through cortical actin remodeling. Nat Commun 6, 5951 (2015).
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Source: Addgene