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URL: http://www.addgene.org/65767
Proper Citation: RRID:Addgene_65767
Insert Name: mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
Organism: Other
Bacterial Resistance: Chloramphenicol
Defining Citation: PMID:26098369
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Comments: Use BsaI sites to insert sgRNA spacer sequence into the vector.
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Source: Addgene
