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URL: http://www.addgene.org/67525
Proper Citation: RRID:Addgene_67525
Insert Name: crumbs
Organism: Drosophila melanogaster
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Size:8904; Vector Backbone:pUAST; Vector Types:Insect Expression, Drosophila; Bacterial Resistance:Ampicillin
Comments: The plasmid contains part of the 5' cDNA of Crb and the 3' part that brings the transmembrane and the intracellular domains of the protein together. The BglII restriction site links the 5' and 3' regions of the cDNA, meaning that the majority of the extracellular part of the Crb protein is missing. The fragment also contains the 5' and 3' UTR regions. BglII restriction site was used to clone a GFP fragment. GFP is inserted between aa 96 and 2122 of crb. Crb contains K89E mutation which does not affect plasmid function.
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No rating or validation information has been found for pUASt- Crb intraGFP.
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Source: Addgene
