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URL: http://www.addgene.org/67880
Proper Citation: RRID:Addgene_67880
Insert Name: dsRed and shRNA to knock down the mouse adra2 receptor
Organism: Gallus gallus
Bacterial Resistance: Chloramphenicol and Ampicillin
Defining Citation: PMID:25706476
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pUC; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The shRNA hairpin sequences were placed into the mir30 site of pPRIME-cmv-dsRed-FF3 (Addgene Plasmid 11664; gift from S. Elledge, Howard Hughes Medical Institute). Inserts in pPRIME were released by SbfI and PacI digestion, and cloned into the PstI and PacI sites in the polylinker of the AAV-genome plasmid pAM-flex (Murray AJ et al.2011 Nat Neurosci 14, p297), to give ITR-cmv-Penhancer/chicken β-actin-dsRED-mir30-shRNA-woodchuck post-translational regulatory sequence (WPRE)-bovine growth hormone polyadenylation signal (pA)-ITR. The sh RNA knocks down mouse adra2a expression.
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No rating or validation information has been found for AAV-actin prom-dsRed-adra2a.1227.shRNA.
No alerts have been found for AAV-actin prom-dsRed-adra2a.1227.shRNA.
Source: Addgene
