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URL: http://www.addgene.org/68437
Proper Citation: RRID:Addgene_68437
Insert Name: TOP1 construct
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:26030444
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pNEB193; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: sgRNA core targets the (ATCTAGATACGACTCACTAT) sequence in the Gluc reporter. Contains a 3' -"accessory domain," comprising the T. thermophilia P4-P6 domain appended with a casette of PP7 stem-loops. Trascription termination is by the U1 snRNA 3' Box system, preceeded by the U2 snRNA sm Box.
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Source: Addgene
