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URL: http://www.addgene.org/71487
Proper Citation: RRID:Addgene_71487
Insert Name: loxP-hyg-cat-loxP
Organism: Other
Bacterial Resistance: Chloramphenicol
Defining Citation: PMID:25779316
Vector Backbone Description: Backbone Marker:Jeffery Murry - Eric Rubin Lab; Vector Backbone:pJM1; Vector Types:Bacterial Expression, Source of loxP-hyg-cat- loxP for PCR amplification.; Bacterial Resistance:Chloramphenicol
Comments: This vector contains a hyg-cat cassette, flanked by loxP sites, that can be used for the construction of recombineeng substrates for M. smegmatis or M. tuberculosis. It contains a 1 bp change (relative to starting plasmid pJM1) at position 2008 (G to C) that eliminate a potential stop codon that could interfere with expression of downstream functions following excision of the cassette from the chromosome by Cre recombinase. The hygromycin resistance gene encodes M13I and I111T variants compared to the NCBI reference [ADL66925.1]. These variants should not interfere with function.
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Source: Addgene
